{"id":105,"date":"2018-02-21T22:45:32","date_gmt":"2018-02-21T21:45:32","guid":{"rendered":"http:\/\/rdre.eu\/?page_id=105"},"modified":"2025-06-18T15:01:36","modified_gmt":"2025-06-18T13:01:36","slug":"bacteria-in-droplets","status":"publish","type":"page","link":"https:\/\/maximeardre.eu\/index.php\/bacteria-in-droplets\/","title":{"rendered":"Bacteria in droplets"},"content":{"rendered":"<p style=\"text-align: left;\">The relationship between the number of cells colonizing a new environment and time for resumption of growth is a subject of long-standing interest. In microbiology this is known as the \u201cinoculum effect.\u201d Its mechanistic basis is unclear with possible explanations ranging from the independent actions of individual cells, to collective actions of populations of cells. Here, we use a millifluidic droplet device in which the growth dynamics of hundreds of populations founded by controlled numbers of <em>Pseudomonas fluorescens<\/em> cells, ranging from a single cell, to one thousand cells, were followed in real time. Our data show that lag phase decreases with inoculum size. The decrease of average lag time and its variance across droplets, as well as lag time distribution shapes, follow predictions of extreme value theory, where the inoculum lag time is determined by the minimum value sampled from the single-cell distribution. Our\u00a0 experimental results show that exit from lag phase depends on strong interactions among cells, consistent with a \u201cleader cell\u201d triggering end of lag phase for the entire population.<\/p>\n<p>Title: <a href=\"https:\/\/academic.oup.com\/microlife\/article\/doi\/10.1093\/femsml\/uqac022\/6794270?guestAccessKey=7e3474e2-ae55-4dd2-a2d6-39246f98653f&amp;utm_source=authortollfreelink&amp;utm_campaign=microlife&amp;utm_medium=email\">A leader cell triggers end of lag phase in populations of <em>Pseudomonas fluorescens<\/em><\/a><\/p>\n<p>Authors : <span class=\"al-author-name-more js-flyout-wrap\">Maxime Ardr\u00e9<span class=\"delimiter\">, <\/span><\/span> <span class=\"al-author-name-more js-flyout-wrap\">Guilhem Doulcier<span class=\"delimiter\">, <\/span><\/span> <span class=\"al-author-name-more js-flyout-wrap\">Naama Brenner<span class=\"delimiter\">, <\/span><\/span><span class=\"al-author-name-more js-flyout-wrap\">Paul B Rainey<\/span><\/p>\n<p>DOI: <span class=\"article-link-citation\"><a href=\"https:\/\/doi.org\/10.1093\/femsml\/uqad040\" data-google-interstitial=\"false\">10.1093\/femsml\/uqad040<\/a><\/span><\/p>\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"962\" height=\"1024\" src=\"http:\/\/maximeardre.eu\/wp-content\/uploads\/2025\/06\/lag-1-962x1024.jpg\" alt=\"\" class=\"wp-image-355\" style=\"width:360px;height:auto\" srcset=\"https:\/\/maximeardre.eu\/wp-content\/uploads\/2025\/06\/lag-1-962x1024.jpg 962w, https:\/\/maximeardre.eu\/wp-content\/uploads\/2025\/06\/lag-1-282x300.jpg 282w, https:\/\/maximeardre.eu\/wp-content\/uploads\/2025\/06\/lag-1-768x818.jpg 768w, https:\/\/maximeardre.eu\/wp-content\/uploads\/2025\/06\/lag-1-1443x1536.jpg 1443w, https:\/\/maximeardre.eu\/wp-content\/uploads\/2025\/06\/lag-1-1924x2048.jpg 1924w\" sizes=\"auto, (max-width: 706px) 89vw, (max-width: 767px) 82vw, 740px\" \/><\/figure><\/div>","protected":false},"excerpt":{"rendered":"<p>The relationship between the number of cells colonizing a new environment and time for resumption of growth is a subject of long-standing interest. In microbiology this is known as the \u201cinoculum effect.\u201d Its mechanistic basis is unclear with possible explanations ranging from the independent actions of individual cells, to collective actions of populations of cells. &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/maximeardre.eu\/index.php\/bacteria-in-droplets\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Bacteria in droplets&#8221;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":111,"parent":0,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-105","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/maximeardre.eu\/index.php\/wp-json\/wp\/v2\/pages\/105","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/maximeardre.eu\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/maximeardre.eu\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/maximeardre.eu\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/maximeardre.eu\/index.php\/wp-json\/wp\/v2\/comments?post=105"}],"version-history":[{"count":9,"href":"https:\/\/maximeardre.eu\/index.php\/wp-json\/wp\/v2\/pages\/105\/revisions"}],"predecessor-version":[{"id":356,"href":"https:\/\/maximeardre.eu\/index.php\/wp-json\/wp\/v2\/pages\/105\/revisions\/356"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/maximeardre.eu\/index.php\/wp-json\/wp\/v2\/media\/111"}],"wp:attachment":[{"href":"https:\/\/maximeardre.eu\/index.php\/wp-json\/wp\/v2\/media?parent=105"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}